|
KMID : 1101420150470030112
|
|
Korean Journal of Clinical Laboratory Science
2015 Volume.47 No. 3 p.112 ~ p.116
|
|
|
Induction of HaCaT Cell Apoptosis by Sodium Nitroprusside
|
|
Park Yu-Ri
Moon Cheol
Lee Pyeong-Jae
|
|
|
Abstract
|
|
|
|
Nitric Oxide (NO) has been known to play important physiological and pathological roles. In this study, Sodium nitroprusside (SNP), NO donor, induced the apoptosis of HaCaT cell, human spontaneous immortal keratinocyte, which was investigated through DAPI staining and cleavage of PARP and caspase-3 protein. However, the expression level of Bip and CHOP, involved in ER stress, was not significantly changed as compared to the control cell group. Recent studies have showed that SIRT1, NAD+-dependent deacetylase, is the key protein that controls cell survival and death. SNP treatment suppressed the SIRT1 gene expression, which indicated that apoptosis induced by SNP could be implicated in SIRT1 down-regulation.
|
|
|
KEYWORD
|
|
|
Apoptosis, Nitric Oxide, HaCaT, SIRT1
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
|
|